Euregional PACT II equipment and expertise

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RWTH Aachen University

Institute for Applied Medical Engineering, Dept. of Experimental Medicine and Immunotherapy, University Hospital of the RWTH Aachen
Fraunhofer-Institute for Molecular Biology and Applied Ecology, Dept. of Pharmaceutical Product Development.

Equipment Application
Molecular biology unit Cloning and sequencing of novel recombinant fusion genes
Antibody unit Generation of antibodies by hybridoma and phages display technology
preGMP fermentation unit Fermentation capacities 1-50 L for bacteria, yeast, mammalian and plant suspension cells
preGMP purification unit Downstream processing of protein extracted from different expression hosts
GMP production unit Two lines 5-500 L for production and purification of proteins for clinical trials
Protein laboratory equipment Analysis of protein by ELISA, Absorption Spectrometry, Fluorometry and Western Blotting, ITC calorimetry to determine ligand binding enthalpy, DSC calorimetry to resolve conformational transitions
Maldi electrospray Peptide mass fingerprinting, MS/MS protein identification
Protein crystallography unit Protein crystallization and X-ray analysis
In vitro protein characterization unit FACSCalibur for binding analyses to living cells, FACSVantage for live cell sorting, Analysis of RT biomolecular interaction using surface Plasmon resonance (Biacore 3000), using confocal microscopy to analyze internalization of fluorescent probes into living cells (high throughput using Ecotec Opera), Apoptosis and cell proliferation assays, immunohistochemistry, cytospins
CriMastro Whole animal (mouse/rat) multiplexed optical imaging using NIR-conjugated proteins

Expertises

Generation of novel recombinant fusion proteins by protein engineering (Immunodiagnostics and –therapeutics), expression of proteins in different hosts (bacteria, yeast, mammalian and plant cells), biochemical characterization of recombinant proteins, characterization of functional activity to living cells or immobilized antigens, characterization of novel immunodiagnostics and –therapeutics in different in vivo models covering inflammation, autoimmune and oncological diseases.

Contact


Prof. Dr. Stefan Barth Partner This e-mail address is being protected from spambots. You need JavaScript enabled to view it